TY - JOUR
KW - Humans
KW - Female
KW - Male
KW - Middle Aged
KW - High-Throughput Nucleotide Sequencing
KW - Metagenome
KW - Retrospective Studies
KW - Capnocytophaga
KW - Eye Infections, Bacterial
KW - Gram-Negative Bacterial Infections
KW - Keratitis
KW - Microbiological Techniques
AU - Gerami Seitzman
AU - Praneetha Thulasi
AU - Armin Hinterwirth
AU - Cindi Chen
AU - Jessica Shantha
AU - Thuy Doan
AB - <p><b>PURPOSE: </b>To report our experience with 2 cases of Capnocytophaga keratitis.</p>

<p><b>METHODS: </b>This is a retrospective study of case reports. We present the clinical presentation, diagnosis, and treatment strategies of 2 patients who presented with Capnocytophaga keratitis.</p>

<p><b>RESULTS: </b>Both patients had risk factors including systemic immune compromise and ocular trauma. Both patients had robust inflammatory keratitis with necrosis. Case 1 demonstrates identification of Capnocytophaga with traditional microbiologic techniques. Case 2 demonstrates the use of unbiased metagenomic deep sequencing for identification of this unusual corneal pathogen.</p>

<p><b>CONCLUSIONS: </b>Capnocytophaga is a rare and aggressive infection. Even when traditional culture identifies the pathogen rapidly, keratitis can progress to perforation. In cases of severe keratitis in which traditional culture methods are unrevealing, metagenomic deep sequencing has potential to provide actionable diagnoses.</p>

BT - Cornea
DA - 2019 Feb
DO - 10.1097/ICO.0000000000001790
IS - 2
J2 - Cornea
LA - eng
N2 - <p><b>PURPOSE: </b>To report our experience with 2 cases of Capnocytophaga keratitis.</p>

<p><b>METHODS: </b>This is a retrospective study of case reports. We present the clinical presentation, diagnosis, and treatment strategies of 2 patients who presented with Capnocytophaga keratitis.</p>

<p><b>RESULTS: </b>Both patients had risk factors including systemic immune compromise and ocular trauma. Both patients had robust inflammatory keratitis with necrosis. Case 1 demonstrates identification of Capnocytophaga with traditional microbiologic techniques. Case 2 demonstrates the use of unbiased metagenomic deep sequencing for identification of this unusual corneal pathogen.</p>

<p><b>CONCLUSIONS: </b>Capnocytophaga is a rare and aggressive infection. Even when traditional culture identifies the pathogen rapidly, keratitis can progress to perforation. In cases of severe keratitis in which traditional culture methods are unrevealing, metagenomic deep sequencing has potential to provide actionable diagnoses.</p>

PY - 2019
SP - 246
EP - 248
T2 - Cornea
TI - Capnocytophaga Keratitis: Clinical Presentation and Use of Metagenomic Deep Sequencing for Diagnosis.
VL - 38
SN - 1536-4798
ER -