Background: Exposure to antibiotics may result in alterations to the composition of intestinal microbiota. However, few trials have been conducted, and observational studies are subject to confounding by indication. We conducted a randomized controlled trial to determine the effect of 3 commonly used pediatric antibiotics on the intestinal microbiome in healthy preschool children.
Methods: Children aged 6-59 months were randomized (1:1:1:1) to a 5-day course of 1 of 3 antibiotics, including amoxicillin (25 mg/kg/d twice-daily doses), azithromycin (10 mg/kg dose on day 1 and then 5 mg/kg once daily for 4 days), cotrimoxazole (240 mg once daily), or placebo. Rectal swabs were obtained at baseline and 5 days after the last dose and were processed using 16S rRNA gene sequencing. The prespecified primary outcome was inverse Simpson's α-diversity index.
Results: Post-treatment Simpson's diversity was significantly different across the 4 arms ( = .003). The mean Simpson's α-diversity among azithromycin-treated children was significantly lower than in placebo-treated children (6.6; 95% confidence interval [CI], 5.5-7.8; vs 9.8; 95% CI, 8.7-10.9; = .0001). Diversity in children treated with amoxicillin (8.3; 95% CI, 7.0-9.6; = .09) or cotrimoxazole (8.3; 95% CI, 8.2-9.7; = .08) was not significantly different than placebo.
Conclusions: Azithromycin affects the composition of the pediatric intestinal microbiome. The effect of amoxicillin and cotrimoxazole on microbiome composition was less clear.
Clinical Trials Registration: clinicaltrials.gov NCT03187834.
BT - Open Forum Infect Dis DA - 2018 Nov DO - 10.1093/ofid/ofy289 IS - 11 J2 - Open Forum Infect Dis LA - eng N2 -Background: Exposure to antibiotics may result in alterations to the composition of intestinal microbiota. However, few trials have been conducted, and observational studies are subject to confounding by indication. We conducted a randomized controlled trial to determine the effect of 3 commonly used pediatric antibiotics on the intestinal microbiome in healthy preschool children.
Methods: Children aged 6-59 months were randomized (1:1:1:1) to a 5-day course of 1 of 3 antibiotics, including amoxicillin (25 mg/kg/d twice-daily doses), azithromycin (10 mg/kg dose on day 1 and then 5 mg/kg once daily for 4 days), cotrimoxazole (240 mg once daily), or placebo. Rectal swabs were obtained at baseline and 5 days after the last dose and were processed using 16S rRNA gene sequencing. The prespecified primary outcome was inverse Simpson's α-diversity index.
Results: Post-treatment Simpson's diversity was significantly different across the 4 arms ( = .003). The mean Simpson's α-diversity among azithromycin-treated children was significantly lower than in placebo-treated children (6.6; 95% confidence interval [CI], 5.5-7.8; vs 9.8; 95% CI, 8.7-10.9; = .0001). Diversity in children treated with amoxicillin (8.3; 95% CI, 7.0-9.6; = .09) or cotrimoxazole (8.3; 95% CI, 8.2-9.7; = .08) was not significantly different than placebo.
Conclusions: Azithromycin affects the composition of the pediatric intestinal microbiome. The effect of amoxicillin and cotrimoxazole on microbiome composition was less clear.
Clinical Trials Registration: clinicaltrials.gov NCT03187834.
PY - 2018 EP - ofy289 T2 - Open Forum Infect Dis TI - Effect of Commonly Used Pediatric Antibiotics on Gut Microbial Diversity in Preschool Children in Burkina Faso: A Randomized Clinical Trial. VL - 5 SN - 2328-8957 ER -