BACKGROUND: Leptospira species are difficult to culture. Thus, when there is suspicion for an infectious etiology to uveitis, bacterial cultures may fail to identify Leptospira. We describe a case of leptospirosis-associated uveitis that evaded culture and molecular assays. DNA sequencing of the aqueous fluid showed the presence of Leptospira spp.
METHODS: Retrospective case review of clinical and laboratory features of a patient with ocular leptospirosis is presented.
RESULTS: DNA sequencing identified the genome of Leptospirosis spp. in the aqueous humor.
CONCLUSION: Metagenomic sequencing, by virtue of its unbiased nature, can be a helpful adjunctive test when a strong clinical suspicion for intraocular infection persists despite negative routine culture and molecular assays.
BT - Ocul Immunol Inflamm DA - 2022 Apr 29 DO - 10.1080/09273948.2022.2067779 J2 - Ocul Immunol Inflamm LA - eng N2 -BACKGROUND: Leptospira species are difficult to culture. Thus, when there is suspicion for an infectious etiology to uveitis, bacterial cultures may fail to identify Leptospira. We describe a case of leptospirosis-associated uveitis that evaded culture and molecular assays. DNA sequencing of the aqueous fluid showed the presence of Leptospira spp.
METHODS: Retrospective case review of clinical and laboratory features of a patient with ocular leptospirosis is presented.
RESULTS: DNA sequencing identified the genome of Leptospirosis spp. in the aqueous humor.
CONCLUSION: Metagenomic sequencing, by virtue of its unbiased nature, can be a helpful adjunctive test when a strong clinical suspicion for intraocular infection persists despite negative routine culture and molecular assays.
PY - 2022 SP - 1 EP - 3 T2 - Ocul Immunol Inflamm TI - Detection of Leptospirosis Genome from the Aqueous Humor of a Patient with Bilateral Uveitis. SN - 1744-5078 ER -