@article {976, title = {Detection of Leptospirosis Genome from the Aqueous Humor of a Patient with Bilateral Uveitis.}, journal = {Ocul Immunol Inflamm}, year = {2022}, month = {2022 Apr 29}, pages = {1-3}, abstract = {

BACKGROUND: Leptospira species are difficult to culture. Thus, when there is suspicion for an infectious etiology to uveitis, bacterial cultures may fail to identify Leptospira. We describe a case of leptospirosis-associated uveitis that evaded culture and molecular assays. DNA sequencing of the aqueous fluid showed the presence of Leptospira spp.

METHODS: Retrospective case review of clinical and laboratory features of a patient with ocular leptospirosis is presented.

RESULTS: DNA sequencing identified the genome of Leptospirosis spp. in the aqueous humor.

CONCLUSION: Metagenomic sequencing, by virtue of its unbiased nature, can be a helpful adjunctive test when a strong clinical suspicion for intraocular infection persists despite negative routine culture and molecular assays.

}, issn = {1744-5078}, doi = {10.1080/09273948.2022.2067779}, author = {Gonzales, John A and Sundararajan, Miel and Qian, Ying and Hwang, Thomas and Doan, Thuy} }