@article {1226, title = {Metagenomic Deep Sequencing for Orbital Inflammatory Disease.}, journal = {Ocul Immunol Inflamm}, year = {2023}, month = {2023 Apr 17}, pages = {1-4}, abstract = {

PURPOSE: Orbital inflammatory disease (OID) is a heterogeneous group of immunologic disorders whose etiology is often non-specific despite routine investigation. In this proof-of-concept study, metagenomic deep sequencing (MDS) is applied to examine host gene expression in two subtypes of OID.

METHODS: Prospectively collected lacrimal gland tissue from patients with OID was processed for MDS. Differential gene expression analysis was performed to evaluate for host transcriptome signatures. Proof-of-concept comparison was made between histologically confirmed samples of idiopathic dacryoadenitis and IgG4-related disease (IgG4-RD).

RESULTS: Twelve genes were identified to be differentially expressed between idiopathic dacryoadenitis and IgG4-RD. Differences in innate humoral immunity gene expression were observed. Several additional genes of interests were also found to be upregulated in idiopathic dacryoadenitis.

CONCLUSIONS: A unique transcriptome signature was found when comparing idiopathic dacryoadenitis to IgG4-RD. This suggests that MDS can identify differentially expressed genes in OID. Such insight could potentially provide a better understanding of host gene expression and the inflammatory pathways involved in OID.

}, issn = {1744-5078}, doi = {10.1080/09273948.2023.2199061}, author = {Vagefi, M Reza and Idowu, Oluwatobi O and Miller, Amanda and Doan, Thuy and Chen, Cindi and Hinterwirth, Armin and Zhong, Lina and Ahmad, Meleha and Ashraf, Davin C and Grob, Seanna R and Kersten, Robert C and Winn, Bryan J} }