Title | Detection of Leptospirosis Genome from the Aqueous Humor of a Patient with Bilateral Uveitis. |
Publication Type | Journal Article |
Year of Publication | 2022 |
Authors | Gonzales JA, Sundararajan M, Qian Y, Hwang T, Doan T |
Journal | Ocul Immunol Inflamm |
Pagination | 1-3 |
Date Published | 2022 Apr 29 |
ISSN | 1744-5078 |
Abstract | BACKGROUND: Leptospira species are difficult to culture. Thus, when there is suspicion for an infectious etiology to uveitis, bacterial cultures may fail to identify Leptospira. We describe a case of leptospirosis-associated uveitis that evaded culture and molecular assays. DNA sequencing of the aqueous fluid showed the presence of Leptospira spp. METHODS: Retrospective case review of clinical and laboratory features of a patient with ocular leptospirosis is presented. RESULTS: DNA sequencing identified the genome of Leptospirosis spp. in the aqueous humor. CONCLUSION: Metagenomic sequencing, by virtue of its unbiased nature, can be a helpful adjunctive test when a strong clinical suspicion for intraocular infection persists despite negative routine culture and molecular assays. |
DOI | 10.1080/09273948.2022.2067779 |
Alternate Journal | Ocul Immunol Inflamm |
PubMed ID | 35486672 |