Title | Effect of Commonly Used Pediatric Antibiotics on Gut Microbial Diversity in Preschool Children in Burkina Faso: A Randomized Clinical Trial. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Oldenburg CE, Sie A, Coulibaly B, Ouermi L, Dah C, Tapsoba C, Bärnighausen T, Ray KJ, Zhong L, Cummings S, Lebas E, Lietman TM, Keenan JD, Doan T |
Journal | Open Forum Infect Dis |
Volume | 5 |
Issue | 11 |
Pagination | ofy289 |
Date Published | 2018 Nov |
ISSN | 2328-8957 |
Abstract | Background: Exposure to antibiotics may result in alterations to the composition of intestinal microbiota. However, few trials have been conducted, and observational studies are subject to confounding by indication. We conducted a randomized controlled trial to determine the effect of 3 commonly used pediatric antibiotics on the intestinal microbiome in healthy preschool children. Methods: Children aged 6-59 months were randomized (1:1:1:1) to a 5-day course of 1 of 3 antibiotics, including amoxicillin (25 mg/kg/d twice-daily doses), azithromycin (10 mg/kg dose on day 1 and then 5 mg/kg once daily for 4 days), cotrimoxazole (240 mg once daily), or placebo. Rectal swabs were obtained at baseline and 5 days after the last dose and were processed using 16S rRNA gene sequencing. The prespecified primary outcome was inverse Simpson's α-diversity index. Results: Post-treatment Simpson's diversity was significantly different across the 4 arms ( = .003). The mean Simpson's α-diversity among azithromycin-treated children was significantly lower than in placebo-treated children (6.6; 95% confidence interval [CI], 5.5-7.8; vs 9.8; 95% CI, 8.7-10.9; = .0001). Diversity in children treated with amoxicillin (8.3; 95% CI, 7.0-9.6; = .09) or cotrimoxazole (8.3; 95% CI, 8.2-9.7; = .08) was not significantly different than placebo. Conclusions: Azithromycin affects the composition of the pediatric intestinal microbiome. The effect of amoxicillin and cotrimoxazole on microbiome composition was less clear. Clinical Trials Registration: clinicaltrials.gov NCT03187834. |
DOI | 10.1093/ofid/ofy289 |
Alternate Journal | Open Forum Infect Dis |
PubMed ID | 30515431 |
PubMed Central ID | PMC6262116 |
Grant List | K08 EY026986 / EY / NEI NIH HHS / United States R25 MH083620 / MH / NIMH NIH HHS / United States |